Alligator blood collection tail clip manual#
Alligator blood collection tail clip full#

Alligator blood collection tail clip manual#

To counter this, manual clot disruption ensures that the clot is challenged during monitoring, preventing primary (platelet) hemostasis from stopping bleeding. A potential limitation in bleeding models is the stochastic nature of hemostasis, which can reduce the reproducibility of the model. Furthermore, it is possible to use both genders, reducing the total number of animals that need to be bred, in adherence with the principles of 3R's. Compared to the TVT survival model, it uses a more humane procedure without compromising sensitivity to pharmacological intervention. Most previously reported blood parameter values were similar to ours and fell within our reference intervals. Although previously reported by others, this study includes additional parameters not yet reported in wild populations. This fast and relatively simple procedure results in consistent and reproducible bleeds. We calculated reference intervals for 48 blood parameters from 120 wild American alligators (Alligator mississippiensis) in South Florida, US. Whatever image you need, we have you more than covered with millions of. Established since 2000, our vast collection of professional stock photos is enriched daily with photos from talented contributors from all over the world. After 40 min, blood loss is quantified by the amount of hemoglobin bled into the saline. Welcome to Dreamstime, worlds largest community for royalty-free photos and stock photography. If no bleeding occurs at 10 min, 20 min, or 30 min post-injury, the clot is challenged gently by wiping the cut twice with a wet gauze swab. After 5 min, the left lateral tail vein is transected using a template guide, the tail is returned to the saline, and all bleeding episodes are monitored and recorded for 40 min while collecting the blood.

Briefly, anesthetized mice are positioned with the tail submerged in temperate saline (37☌) and dosed with the test compound in the right lateral tail vein. Chemists in Louisiana found that blood from the American alligator can successfully destroy 23 strains of bacteria, including strains known to be resistant to antibiotics.

Alligator blood collection tail clip full#

To avoid using survival as an endpoint, we developed a TVT model establishing blood loss and bleeding time as endpoints and full anesthesia during the entire experiment. The tail vein transection (TVT) survival model has been preferred in many settings due to sensitivity to clinically relevant doses of FVIII, whereas other established models, such as the tail clip model, require higher levels of procoagulant compounds. The tail vein transection (TVT) survival model has been preferred in many settings due to sensitivity to clinically relevant doses of FVIII, whereas other established models, such as the tail clip model, require higher levels of procoagulant compounds. Tail bleeding models are important tools in hemophilia research, specifically for the assessment of procoagulant effects.